Journal: International Journal of Nanomedicine

Article Title: Combined Placental Mesenchymal Stem Cells with Guided Nanoparticles Effective Against Diabetic Nephropathy in Mouse Model

doi: 10.2147/IJN.S446733

Figure Lengend Snippet: Internalization of Nanoparticles (NPs) into Placental Mesenchymal Stem Cells (PL-MSCs). ( A ) MSCs stained with Prussian Blue to highlight iron (Fe) content. Scale bar = 50 µm. ( B ) Quantification of Fe concentration using Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) (n=3). ( C ) Assessment of NP-induced cytotoxicity in MSCs labeled with NPs at varying concentrations for 24 h (n=4). ( D ) Measurement of β-galactosidase levels in NP-labeled MSCs using ELISA (n=4). ( E ) Proliferation rates of cells labeled with NPs at different concentrations (n=5). ( F and G ) Transmission Electron Microscopy (TEM) images showing NPs internalized into an MSC at a concentration of 50 µg/mL. The red arrow indicates NP. Data are presented as mean ± S.E.M. P -values < 0.05 were considered statistically significant, denoted as * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The supernatants were collected for β-galactosidase analysis using a human β-galactosidase ELISA kit (Molbio, Shanghai, China), in accordance with the manufacturer’s protocol.

Techniques: Staining, Concentration Assay, Clinical Proteomics, Spectroscopy, Labeling, Enzyme-linked Immunosorbent Assay, Transmission Assay, Electron Microscopy

Journal: ACS Omega

Article Title: Intracellular Delivery of β-Galactosidase Enzyme Using Arginase-Responsive Dextran Sulfate/Poly- l -arginine Capsule for Lysosomal Storage Disorder

doi: 10.1021/acsomega.7b01230

Figure Lengend Snippet: Cytotoxicity studies of bare DS/PA capsules with (a) R201C, (b) SV, (c) HeLa, and (d) wild-type mouse fibroblast cell lines.

Article Snippet: SV (β-galactosidase gene-deficient mouse fibroblast, JCRB1207) and R201C (deficient human β-galactosidase gene-introduced mouse fibroblast, JCRB1199), were purchased from the Japanese Collection of Research Bioresources cell bank.

Techniques: Capsules

Journal: ACS Omega

Article Title: Intracellular Delivery of β-Galactosidase Enzyme Using Arginase-Responsive Dextran Sulfate/Poly- l -arginine Capsule for Lysosomal Storage Disorder

doi: 10.1021/acsomega.7b01230

Figure Lengend Snippet: Uptake studies of DS/PA capsules with (a) SV, (b) R201C, (c) wild-type mouse fibroblast, and (d) HeLa cells after 12 h. (Note: Inset figures show Z-stack images of corresponding cells.)

Article Snippet: SV (β-galactosidase gene-deficient mouse fibroblast, JCRB1207) and R201C (deficient human β-galactosidase gene-introduced mouse fibroblast, JCRB1199), were purchased from the Japanese Collection of Research Bioresources cell bank.

Techniques: Capsules

Journal: ACS Omega

Article Title: Intracellular Delivery of β-Galactosidase Enzyme Using Arginase-Responsive Dextran Sulfate/Poly- l -arginine Capsule for Lysosomal Storage Disorder

doi: 10.1021/acsomega.7b01230

Figure Lengend Snippet: GM1 ganglioside levels in R201C cells (deficient human β-gal-gene-introduced mouse fibroblast) treated with free β-gal enzyme equivalent to the enzyme loaded in the capsules (top panel, a, c) and 50 β-gal-DS/PA capsules/cells (bottom panel, a) and 100 β-gal-DS/PA capsules/cells (bottom panel, c) at different time points (6, 12, and 24 h). FITC-cholera toxin B was used as a detection probe for GM1 ganglioside accumulation in culture fibroblasts. (b) and (d) represent the corresponding fluorescence intensity. Note: Control is untreated cells, and negative control is cells treated with bare DS/PA capsules (50 and 100 capsules/cell).

Article Snippet: SV (β-galactosidase gene-deficient mouse fibroblast, JCRB1207) and R201C (deficient human β-galactosidase gene-introduced mouse fibroblast, JCRB1199), were purchased from the Japanese Collection of Research Bioresources cell bank.

Techniques: Capsules, Fluorescence, Control, Negative Control

Journal: ACS Omega

Article Title: Intracellular Delivery of β-Galactosidase Enzyme Using Arginase-Responsive Dextran Sulfate/Poly- l -arginine Capsule for Lysosomal Storage Disorder

doi: 10.1021/acsomega.7b01230

Figure Lengend Snippet: GM1 ganglioside levels in treated (50 capsules/cell) and untreated R201C, SV, and wild-type mouse fibroblast cell line.

Article Snippet: SV (β-galactosidase gene-deficient mouse fibroblast, JCRB1207) and R201C (deficient human β-galactosidase gene-introduced mouse fibroblast, JCRB1199), were purchased from the Japanese Collection of Research Bioresources cell bank.

Techniques: Capsules